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remove_cells_w_zero_or_twoplus_grnas
to
run_qc()
, which is relevant for low-MOI analyses withcontrol_group = "nt_cells"
.If set to
TRUE
, then cells with zero or two or more gRNAs are removed from the analysis, as before.If set to
FALSE
, then cells with multiple gRNAs are kept in the analysis ifthey contain at most one targeting gRNA. For these cells, a perturbation identity
can be unambiguously defined, and their inclusion increases the number of cells
available for downstream analysis.
analysis have two additional columns called
n_trt
andn_cntrl
, giving thenumber of cells used in the treatment and control groups, respectively.
construct_cis_pairs()
thatgrna_target_data_frame
has columns
chr
,start
, andend
, resolving this issue.