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Merge pull request #836 from daisymut/sammyseq
Added test data for SAMMYseq data, mapping on part of human chr22
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# ![nfcore/test-datasets](docs/images/test-datasets_logo.png) | ||
Test data to be used for automated testing with the nf-core pipelines | ||
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> ⚠️ **Do not merge your test data to `master`! Each pipeline has a dedicated branch (and a special one for modules)** | ||
# test-datasets: `sammyseq` | ||
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## Introduction | ||
This branch contains data to be used for automated testing with the [nf-core/sammyseq](https://github.com/daisymut/sammyseq) pipeline. | ||
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nf-core is a collection of high quality Nextflow pipelines. This repository contains various files for CI and unit testing of nf-core pipelines and infrastructure. | ||
## Content of this repository | ||
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The principle for nf-core test data is as small as possible, as large as necessary. Please see the [guidelines](https://nf-co.re/docs/contributing/test_data_guidelines) for more detailed information. Always ask for guidance on the [nf-core slack](https://nf-co.re/join) before adding new test data. | ||
`testdata/CTRL004_S*_chr22only.fq.gz`: Human fibroblast single-end test data for pipeline sub-sampled to map on part of chr22. | ||
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## Documentation | ||
## Minimal test dataset origin | ||
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nf-core/test-datasets comes with documentation in the `docs/` directory: | ||
_H. sapiens_ fibroblast, 50bp single-end 3-fraction SAMMY-seq sequences was obtained from: | ||
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01. [Add a new test dataset](https://github.com/nf-core/test-datasets/blob/master/docs/ADD_NEW_DATA.md) | ||
02. [Use an existing test dataset](https://github.com/nf-core/test-datasets/blob/master/docs/USE_EXISTING_DATA.md) | ||
> Sebestyén, E., Marullo, F., Lucini, F. et al. SAMMY-seq reveals early alteration of heterochromatin and deregulation of bivalent genes in Hutchinson-Gilford Progeria Syndrome. Nat Commun 11, 6274 (2020). https://doi.org/10.1038/s41467-020-20048-9. [Pubmed](https://pubmed.ncbi.nlm.nih.gov/33293552/) [GEO](https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE118633) | ||
## Downloading test data | ||
### Sampling information | ||
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Due the large number of large files in this repository for each pipeline, we highly recommend cloning only the branches you would use. | ||
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```bash | ||
git clone <url> --single-branch --branch <pipeline/modules/branch_name> | ||
``` | ||
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To subsequently clone other branches[^1] | ||
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```bash | ||
git remote set-branches --add origin [remote-branch] | ||
git fetch | ||
``` | ||
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## Support | ||
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For further information or help, don't hesitate to get in touch on our [Slack organisation](https://nf-co.re/join/slack) (a tool for instant messaging). | ||
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[^1]: From [stackoverflow](https://stackoverflow.com/a/60846265/11502856) | ||
| GEO_sample | run_accession | read_count | SRA_experiment | sample_title | | ||
| ---------- | ------------- | ---------- | -------------- | -------------------- | | ||
| GSM3335763 | SRR7610706 | 78683296 | SRX4475555 | CTRL004 SAMMY-seq S2 | | ||
| GSM3335764 | SRR7610707 | 60438514 | SRX4475554 | CTRL004 SAMMY-seq S3 | | ||
| GSM3335765 | SRR7610708 | 54864540 | SRX4475553 | CTRL004 SAMMY-seq S4 | |
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