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pUC19-SmaI-a.gb
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LOCUS pUC19 2778 bp ds-DNA circular 18-OCT-2012
DEFINITION Cloning vector pUC19c, complete sequence.
ACCESSION L09137 X02514
VERSION L09137.2 GI:20141090
KEYWORDS .
SOURCE Cloning vector pUC19c
ORGANISM Cloning vector pUC19c other sequences; artificial sequences;
vectors.
REFERENCE 1 (bases 1 to 2686)
AUTHORS Yanisch-Perron,C., Vieira,J. and Messing,J.
TITLE Improved M13 phage cloning vectors and host strains: nucleotide
sequences of the M13mp18 and pUC19 vectors
JOURNAL Gene 33 (1), 103-119 (1985)
PUBMED 2985470
REFERENCE 2 (bases 1 to 2686)
AUTHORS Chambers,S.P., Prior,S.E., Barstow,D.A. and Minton,N.P.
TITLE The pMTL nic- cloning vectors. I. Improved pUC polylinker regions
to facilitate the use of sonicated DNA for nucleotide sequencing
JOURNAL Gene 68 (1), 139-149 (1988)
PUBMED 2851488
REFERENCE 3 (bases 1 to 2686)
AUTHORS Gilbert,W.
TITLE Obtained from VecBase 3.0
JOURNAL Unpublished
REFERENCE 4 (bases 1 to 2686)
AUTHORS Messing,J.
TITLE Direct Submission
JOURNAL Submitted (27-APR-1993) Department of Biochemistry, University of
Minnesota, St. Paul, MN 55108, USA
REFERENCE 5 (bases 1 to 2686)
AUTHORS Messing,J.
TITLE Direct Submission
JOURNAL Submitted (11-APR-2002) Rutgers, The State University of New
Jersey, Waksman Institute of Microbiology, 190 Frelinghuysen Road,
Piscataway, NJ 08854-8020, USA
REMARK Sequence update by submitter
COMMENT On Apr 11, 2002 this sequence version replaced gi:209213. These
data and their annotation were supplied to GenBank by Will Gilbert
under the auspices of the GenBank Currator Program. pUC19c -
Cloning vector (beta-galactosidase mRNA on complementary strand)
ENTRY PUC19C #TYPE DNA CIRCULAR TITLE pUC19c -
Cloning vector (beta-galactosidase mRNA on
complementary strand) DATE 03-FEB-1986 #sequence
16-DEC-1986 ACCESSION VB0033 SOURCE artificial COLLECTION ATCC
37254 REFERENCE #number #authors Norrander J., Kempe T.,
Messing J. #journal Gene (1983) 26: 101-106 REFERENCE #number
1 #authors Yanisch-Perron C., Vieira J., Messing J. #journal
Gene (1985) 33: 103-119 #comment shows the complete compiled
sequence REFERENCE #number 2 #authors Chambers,S.P., et al.
#journal Gene (1988) 68: 139-149 #describes mutation at nt1308
and its effect on copy number REFERENCE #number #authors
Pouwels P.H., Enger-Valk B.E., Brammar W.J. #book Cloning
Vectors, Elsvier 1985 and supplements #comment vector I-A-iv-20
COMMENT This Sequence was obtained 3-MAR-1986 from J. Messing,
Waksman Institute, NJ on floppy disk. Revised 16-DEC-1986 by
F. Pfeiffer: 1062/3 'AT' to 'TA' to match revised sequence of
PBR322 The beta-galactosidase mRNA sequence including the
multiple cloning site of M13mp19 is on the strand complementary
to that shown. KEYWORDS CROSSREFERENCE #complement
VecBase(3):pUC19 #prerevised GenBank(50):M11662,
EMBL(11):ARPuc19 #parent VecBase(3):pUC13,
VecBase(3):M13mp19, VecSource(3):bGal19 PARENT Features of
pUC19c (2686 bp) residue source 1- 137 2074-2210
pBR322 138- 237 2252-2351 pBR322 238- 395 1461-1304 (c)
Lac-Operon 396- 452 57- 1 (c) polylinker of M13mp19
455- 682 1298-1071 (c) Lac-Operon 683-2686 2352-4355 pBR322
Conflict (cfl) and Mutations (mut): pUC19c source mut
1308 A G 2977 pBR322 linked to increased copy number mut
1942 A G 3611 pBR322 mut 2243 T C 3912 pBR322 FEATURE
1629-2417 789-1 (c) Ap-R; b-lactamase POLYLINKER
HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-KpnI-SacI-EcoRI SELECTION
#resistance Ap #indicator beta-galactosidase SUMMARY pUC19c
#length 2686 #checksum 4465.
COMMENT
COMMENT ApEinfo:methylated:1
FEATURES Location/Qualifiers
source 1..414
/organism="Cloning vector pUC19c"
/mol_type="genomic DNA"
/db_xref="taxon:174689"
/label=source:Cloning vector pUC19c
/ApEinfo_fwdcolor=pink
/ApEinfo_revcolor=pink
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
source 507..2778
/organism="Cloning vector pUC19c"
/mol_type="genomic DNA"
/db_xref="taxon:174689"
/label=source:Cloning vector pUC19c(1)
/ApEinfo_label=source:Cloning vector pUC19c
/ApEinfo_fwdcolor=pink
/ApEinfo_revcolor=pink
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccGGATCC
421 tcatctacta tcatcgtagc gtactgatct attctgctgc tcatcatcgg tactctctat
481 aattatatat atatgcgcgt GGATCCgggg atcctctaga gtcgacctgc aggcatgcaa
541 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
601 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
661 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
721 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
781 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
841 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
901 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
961 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
1021 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
1081 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
1141 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
1201 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
1261 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
1321 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
1381 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
1441 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
1501 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
1561 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
1621 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
1681 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
1741 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
1801 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
1861 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
1921 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
1981 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
2041 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
2101 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
2161 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
2221 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
2281 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
2341 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
2401 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
2461 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
2521 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
2581 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
2641 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
2701 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
2761 tcacgaggcc ctttcgtc
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