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pCAPs-pSU0.gb
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LOCUS AJ001614_1 5762 bp ds-DNA circular 11-JUL-2012
DEFINITION Escherichia coli plasmid pCAPs.
ACCESSION AJ001614
VERSION AJ001614.1 GI:2769261
KEYWORDS ampicillin resistance; beta-lactamase; bla gene; catabolite gene
activator protein; crp gene; cyclic amp receptor protein.
SOURCE Escherichia coli
ORGANISM Escherichia coli Bacteria; Proteobacteria; Gammaproteobacteria;
Enterobacteriales; Enterobacteriaceae; Escherichia.
REFERENCE 1
AUTHORS Schlieper,D., von Wilcken-Bergmann,B., Schmidt,M., Sobek,H. and
Muller-Hill,B.
TITLE A positive selection vector for cloning of long polymerase chain
reaction fragments based on a lethal mutant of the crp gene of
Escherichia coli
JOURNAL Anal. Biochem. 257 (2), 203-209 (1998)
PUBMED 9514792
REFERENCE 2 (bases 1 to 3128)
AUTHORS Schlieper,D.
TITLE Direct Submission
JOURNAL Submitted (15-SEP-1997) Schlieper D., Institute of Genetics,
University of Cologne, Weyertal 121, Cologne, 50931, GERMANY
COMMENT
COMMENT ApEinfo:methylated:1
FEATURES Location/Qualifiers
gene complement(315..947)
/gene="crp"
/label=crp
CDS complement(315..947)
/product="catabolite gene activator protein"
/codon_start="1"
/transl_table="11"
/note="artificially mutated crp; cyclic AMP receptor
protein"
/db_xref="GI:2769262"
/experiment="experimental evidence, no additional details
recorded"
/translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
K
GSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKKF
R
QLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVSGRIAQTLLNLAKQPDAMTHPD
G MQIKITRQEIGQIVGCSRQTVGRILKMLEDQNLISAHGKTIVVYGTR"
/gene="crp"
/protein_id="CAA04867.1"
/label=catabolite gene activator protein
gene complement(4706..5566)
/gene="Amp"
/label=Amp
gene complement(2057..2860)
/gene="ura3"
/label=ura3
CDS complement(2057..2860)
/product="orotidine-5'-phosphate decarboxylase"
/codon_start="1"
/transl_table="11"
/db_xref="GI:84570490"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
L
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKL
Q
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTG
E
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVD
D VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
/gene="ura3"
/protein_id="BAE72675.1"
/label=orotidine-5'-phosphate decarboxylase
rep_origin complement(1295..1977)
/label=ColE1 origin
gene complement(join(1979..2649,2698..3086))
/label=URA3
misc_feature complement(3109..4560)
/label=2micron
misc_feature complement(2669..2669)
/label=c>t to remove EcoRV
misc_feature 1278..1278
/label=G or C pCAPs/pSU0
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatagatc ctgaggatcg gggtgataaa tcagtctgcg ccacatcggg ggaaacaaaa
241 tggcgcgaga tctaaaaaaa aaggctccaa aaggagcctt tcgcgctacc aggtaacgcg
301 ccactccgac gggattaacg agtgccgtaa acgacgatgg ttttaccgtg tgcggagatc
361 aggttctgat cctcgagcat cttaagaatt cgtcccacgg tttgtctaga gcagccgaca
421 atctggccaa tttcctgacg ggtaattttg atttgcatgc cgtccgggtg agtcatagcg
481 tctggttgtt ttgccagatt cagcagagtc tgtgcaatgc ggccgctgac gtcgaggaac
541 gccaggttgc ccactttctc actagtgacc tgcagccggc gcgccatctg tgcagacaaa
601 cgcatcagga tatccggatt tacctgaatc aattggcgaa attttttgta cgaaatttca
661 gccacttcac aggcggtttt cgcacgtacc catgcgctac gttcctggcc ctcttcaaac
721 aggcccagtt cgccaataaa atcaccctga ttcagatagg agaggatcat ttctttaccc
781 tcttcgtctt tgatcagcac tgccacagag cctttaacga tgtagtacag cgtttccgct
841 ttttcaccct ggtgaataag cgtgctcttg gatgggtact tatgaatgtg gcaatgagac
901 aagaaccatt cgagagtagg atccgtttga ggtttaccaa gtaccataag atccttaaat
961 ttttattatc tagctagatg ataatattat atcaagaatt gtacctgaaa gcaaataaat
1021 tttttatctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
1081 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
1141 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
1201 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
1261 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
1321 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
1381 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
1441 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
1501 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
1561 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
1621 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
1681 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
1741 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
1801 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
1861 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
1921 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgaggg
1981 gtaataactg atataattaa attgaagctc taatttgtga gtttagtata catgcattta
2041 cttataatac agttttttag ttttgctggc cgcatcttct caaatatgct tcccagcctg
2101 cttttctgta acgttcaccc tctaccttag catcccttcc ctttgcaaat agtcctcttc
2161 caacaataat aatgtcagat cctgtagaga ccacatcatc cacggttcta tactgttgac
2221 ccaatgcgtc tcccttgtca tctaaaccca caccgggtgt cataatcaac caatcgtaac
2281 cttcatctct tccacccatg tctctttgag caataaagcc gataacaaaa tctttgtcgc
2341 tcttcgcaat gtcaacagta cccttagtat attctccagt agatagggag cccttgcatg
2401 acaattctgc taacatcaaa aggcctctag gttcctttgt tacttcttct gccgcctgct
2461 tcaaaccgct aacaatacct gggcccacca caccgtgtgc attcgtaatg tctgcccatt
2521 ctgctattct gtatacaccc gcagagtact gcaatttgac tgtattacca atgtcagcaa
2581 attttctgtc ttcgaagagt aaaaaattgt acttggcgga taatgccttt agcggcttaa
2641 ctgtgccctc catggaaaaa tcagtcaaaa tatccacatg tgtttttagt aaacaaattt
2701 tgggacctaa tgcttcaact aactccagta attccttggt ggtacgaaca tccaatgaag
2761 cacacaagtt tgtttgcttt tcgtgcatga tattaaatag cttggcagca acaggactag
2821 gatgagtagc agcacgttcc ttatatgtag ctttcgacat gatttatctt cgtttcctgc
2881 aggtttttgt tctgtgcagt tgggttaaga atactgggca atttcatgtt tcttcaacac
2941 tacatatgcg tatatatacc aatctaagtc tgtgctcctt ccttcgttct tccttctgtt
3001 cggagattac cgaatcaaaa aaatttcaaa gaaaccgaaa tcaaaaaaaa gaataaaaaa
3061 aaaatgatga attgaattga aaagctagct tatcgatgat aagctgtcaa agatgagaat
3121 taattccacg gactatagac tatactagat actccgtcta ctgtacgata cacttccgct
3181 caggtccttg tcctttaacg aggccttacc actcttttgt tactctattg atccagctca
3241 gcaaaggcag tgtgatctaa gattctatct tcgcgatgta gtaaaactag ctagaccgag
3301 aaagagacta gaaatgcaaa aggcacttct acaatggctg ccatcattat tatccgatgt
3361 gacgctgcag cttctcaatg atattcgaat acgctttgag gagatacagc ctaatatccg
3421 acaaactgtt ttacagattt acgatcgtac ttgttaccca tcattgaatt ttgaacatcc
3481 gaacctggga gttttccctg aaacagatag tatatttgaa cctgtataat aatatatagt
3541 ctagcgcttt acggaagaca atgtatgtat ttcggttcct ggagaaacta ttgcatctat
3601 tgcataggta atcttgcacg tcgcatcccc ggttcatttt ctgcgtttcc atcttgcact
3661 tcaatagcat atctttgtta acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa
3721 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg
3781 caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa
3841 tgcaacgcga cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
3901 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
3961 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
4021 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
4081 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
4141 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
4201 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
4261 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
4321 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
4381 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
4441 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
4501 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
4561 gggaagctcc accccggttg ataatcagaa aagccccaaa aacaggaaga ttattatcaa
4621 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
4681 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
4741 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
4801 tacgggagcg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
4861 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
4921 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
4981 gttcgccagt taatagtttg cgcaacgttg ttggcattgc tacaggcatc gtggtgtcac
5041 tctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
5101 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
5161 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
5221 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
5281 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aatagtgtat
5341 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
5401 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
5461 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
5521 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
5581 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
5641 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgcta
5701 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
5761 tc
//