This script picks introns flanking the circRNAs for potential binding and conservation analysis purposes. CircRNAs are a novel form of non-coding RNAs with growing attention from the scientific community.
Copy and paste the following commands in your terminal:
git clone https://github.com/alexandruioanvoda/IntronPicker
cd ./IntronPicker
chmod +x intron_picker.sh
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Download a BED6 file containing all the intronic annotations from UCSC Tables (https://genome.ucsc.edu/cgi-bin/hgTables)
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Copy the BED containing all your circRNA annotations (or any annotations of which you want the find the flanking introns of) in the IntronPicker folder
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Run IntronPicker by typing this in terminal (after navigating into the folder):
./intron_picker.sh circRNAs.bed intron_intervals.bed
- Output is in the same folder