diff --git a/.meta.toml b/.meta.toml
index 8814ecb..da6b5b9 100644
--- a/.meta.toml
+++ b/.meta.toml
@@ -26,3 +26,20 @@ additional-rules = [
 
 [tox]
 use-flake8 = true
+testenv-deps = [
+    "# The universal2 environment is for Python on macOS built with",
+    "# universal2 mode instead of architecture-specific. These dependencies",
+    "# must be installed separately, zc.buildout is incompatible with the",
+    "# universal2 mode and cannot install them itself.",
+    "universal2: -c {toxinidir}/constraints.txt",
+    "universal2: zope.interface",
+    "universal2: zope.security",
+    "universal2: zope.container",
+    "universal2: Persistence",
+    "universal2: Acquisition",
+    "universal2: AccessControl",
+    "universal2: zodbpickle",
+    "universal2: charset_normalizer",
+    "universal2: MarkupSafe",
+    "universal2: zope.testrunner",
+    ]
diff --git a/tox.ini b/tox.ini
index aee1c0d..eb3d518 100644
--- a/tox.ini
+++ b/tox.ini
@@ -18,6 +18,21 @@ deps =
     setuptools < 69
     zc.buildout >= 3.0.1
     wheel > 0.37
+    # The universal2 environment is for Python on macOS built with
+    # universal2 mode instead of architecture-specific. These dependencies
+    # must be installed separately, zc.buildout is incompatible with the
+    # universal2 mode and cannot install them itself.
+    universal2: -c {toxinidir}/constraints.txt
+    universal2: zope.interface
+    universal2: zope.security
+    universal2: zope.container
+    universal2: Persistence
+    universal2: Acquisition
+    universal2: AccessControl
+    universal2: zodbpickle
+    universal2: charset_normalizer
+    universal2: MarkupSafe
+    universal2: zope.testrunner
 setenv =
     py312: VIRTUALENV_PIP=23.1.2
     py312: PIP_REQUIRE_VIRTUALENV=0