Issues using CellChat for Rat

[shiave]

Hi, I'm trying to run CellChat on one Seurat object with 2 conditions, with rat as the subject. I am using the database that was created for rat specifically for this package from this paper: at this github link.

I updated the database as shown below:

interaction_input <- CellChatDB$interaction
complex_input <- CellChatDB$complex
cofactor_input <- CellChatDB$cofactor
geneInfo <- CellChatDB$geneInfo
CellChatDB.rat <- list(
    interaction = interaction_input,
    complex = complex_input,
    cofactor = cofactor_input,
    geneInfo = geneInfo
)

CellChatDB <- CellChatDB.rat

then I read in my seurat object and did the following:

data.input <- seurat_object[["SCT"]]$data
labels <- Idents(seurat_object)
meta <- data.frame(labels = labels, row.names = names(labels)) # create a dataframe of the cell labels
colnames([email protected])[colnames([email protected]) == "sample"] <- "samples"

[1] "Create a CellChat object from a Seurat object"
The meta.data slot in the Seurat object is used as cell meta information
Warning in createCellChat(object = seurat_object, group.by = "ident", assay = "SCT") :
The 'meta$samples' is not a factor. We now force it as a factor!

Set cell identities for the new CellChat object
The cell groups used for CellChat analysis are Oligodendrocytes, Excitatory Neurons, Inhibitory Neurons, Oligodendrocyte Precursors, Microglia, Astrocytes, Endothelial

cellChat <- createCellChat(object = seurat_object, group.by = "ident", assay = "SCT")
CellChatDB.use <- subsetDB(CellChatDB, search = "Secreted Signaling", key = "annotation")
cellChat@DB <- CellChatDB.use

When I get here: cellChat <- subsetData(cellChat) # This step is necessary even if using the whole database
I get this error:
Issue identified!! Please check the official Gene Symbol of the following genes:
NA
Issue identified!! Please check the official Gene Symbol of the following genes:
NA

Can you provide any clarity about what SubsetData does exactly?