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PRESTA

PRE-processing of Sequencing daTA

PRESTA handles sequencing data produced from a large scale NGS Core facility. It leverages a reliable distributed task queue to create a messages-driven automation system. Presta is focused on real-time operation, but supports scheduling as well.

Managing tasks and messages, PRESTA is so able to automatically orchestrate format conversion, demultiplexing, quality checks, LIMS update and data preparation for on-site storage or delivery to external sites.

PRESTA is part of a fully automated infrastructure to support bioinformatics analysis of sequencing data.

Presta_architecture

Tasks currently supported:

  • Monitoring of the state of an Illumina rundir
  • Illumina bcl2fastq software execution at the end of the sequencer's run
  • Production of a detailed quality report on sequencing data coming from the previous step
  • Updating the activities details on the LIMS portal
  • Data delivery to a remote FTP folder, to an iRODS collection, to a local/remote filesystem path or to a removable device.
  • Querying the LIMS portal to retrieve details about the data produced

Requirements: