diff --git a/tornado/test/httpclient_test.py b/tornado/test/httpclient_test.py
index 793dce13c5..1eb758b555 100644
--- a/tornado/test/httpclient_test.py
+++ b/tornado/test/httpclient_test.py
@@ -691,11 +691,13 @@ def test_response_times(self):
         response = self.fetch("/hello")
         response.rethrow()
         self.assertIsNotNone(response.request_time)
+        assert response.request_time is not None  # for mypy
         self.assertGreaterEqual(response.request_time, 0)
         self.assertLess(response.request_time, 1.0)
         # A very crude check to make sure that start_time is based on
         # wall time and not the monotonic clock.
         self.assertIsNotNone(response.start_time)
+        assert response.start_time is not None  # for mypy
         self.assertLess(abs(response.start_time - start_time), 1.0)
 
         for k, v in response.time_info.items():
diff --git a/tornado/test/web_test.py b/tornado/test/web_test.py
index 2fa146d130..ecd7263566 100644
--- a/tornado/test/web_test.py
+++ b/tornado/test/web_test.py
@@ -132,6 +132,7 @@ def test_cookie_tampering_future_timestamp(self):
         cookie = handler._cookies["foo"]
         match = re.match(rb"12345678\|([0-9]+)\|([0-9a-f]+)", cookie)
         self.assertIsNotNone(match)
+        assert match is not None  # for mypy
         timestamp = match.group(1)
         sig = match.group(2)
         self.assertEqual(
@@ -402,8 +403,10 @@ def test_set_cookie_expires_days(self):
         response = self.fetch("/set_expires_days")
         header = response.headers.get("Set-Cookie")
         self.assertIsNotNone(header)
+        assert header is not None  # for mypy
         match = re.match("foo=bar; expires=(?P<expires>.+); Path=/", header)
         self.assertIsNotNone(match)
+        assert match is not None  # for mypy
 
         expires = datetime.datetime.now(datetime.timezone.utc) + datetime.timedelta(
             days=10