diff --git a/.github/workflows/python-package.yml b/.github/workflows/python-package.yml
index 559779617..b75bd6671 100644
--- a/.github/workflows/python-package.yml
+++ b/.github/workflows/python-package.yml
@@ -30,7 +30,7 @@ jobs:
         sudo apt update
         sudo apt-get install -y libopenslide-dev openslide-tools libopenjp2-7 libopenjp2-tools
         python -m pip install --upgrade pip
-        python -m pip install ruff==0.6.2 pytest pytest-cov pytest-runner
+        python -m pip install ruff==0.6.5 pytest pytest-cov pytest-runner
         pip install -r requirements/requirements.txt
     - name: Cache tiatoolbox static assets
       uses: actions/cache@v3
diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index 5c608239e..b2a53033d 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -60,7 +60,7 @@ repos:
       - id: rst-inline-touching-normal # Detect mistake of inline code touching normal text in rst.
   - repo: https://github.com/astral-sh/ruff-pre-commit
     # Ruff version.
-    rev: v0.6.2
+    rev: v0.6.5
     hooks:
       - id: ruff
         args: [--fix, --exit-non-zero-on-fix]
diff --git a/requirements/requirements_dev.txt b/requirements/requirements_dev.txt
index a8392667e..689bb6d44 100644
--- a/requirements/requirements_dev.txt
+++ b/requirements/requirements_dev.txt
@@ -11,7 +11,7 @@ pytest>=7.2.0
 pytest-cov>=4.0.0
 pytest-runner>=6.0
 pytest-xdist[psutil]
-ruff==0.6.2  # This will be updated by pre-commit bot to latest version
+ruff==0.6.5  # This will be updated by pre-commit bot to latest version
 toml>=0.10.2
 twine>=4.0.1
 wheel>=0.37.1