diff --git a/.gitignore b/.gitignore
index 1c7130929..a192542d6 100644
--- a/.gitignore
+++ b/.gitignore
@@ -39,7 +39,6 @@ pip-delete-this-directory.txt
 
 # Unit test / coverage reports
 htmlcov/
-.tox/
 .coverage
 .coverage.*
 .cache
diff --git a/CONTRIBUTING.rst b/CONTRIBUTING.rst
index 1ff073da9..1b8f7a3e9 100644
--- a/CONTRIBUTING.rst
+++ b/CONTRIBUTING.rst
@@ -77,13 +77,12 @@ Ready to contribute? Here's how to set up ``tiatoolbox`` for local development.
    Now you can make your changes locally.
 
 5. When you're done making changes, check that your changes pass flake8 and the
-   tests, including testing other Python versions with tox::
+   tests::
 
     $ flake8 tiatoolbox tests
     $ python setup.py test or pytest
-    $ tox
 
-   To get flake8 and tox, just pip install them into your virtualenv.
+   To get flake8, just pip install them into your virtualenv.
 
 6. Commit your changes and push your branch to GitHub::
 
diff --git a/Makefile b/Makefile
index 21822be50..bbf78b91c 100644
--- a/Makefile
+++ b/Makefile
@@ -42,7 +42,6 @@ clean-pyc: ## remove Python file artifacts
 	find . -name '__pycache__' -exec rm -fr {} +
 
 clean-test: ## remove test and coverage artifacts
-	rm -fr .tox/
 	rm -f .coverage
 	rm -fr htmlcov/
 	rm -fr .pytest_cache
@@ -62,9 +61,6 @@ lint: ## check style with flake8
 test: ## run tests quickly with the default Python
 	pytest
 
-test-all: ## run tests on every Python version with tox
-	tox
-
 coverage: ## check code coverage quickly with the default Python
 	pytest  --cov=tiatoolbox --cov-report=term --cov-report=html --cov-report=xml
 	$(BROWSER) htmlcov/index.html
diff --git a/pyproject.toml b/pyproject.toml
index 14ec1da83..c6924538e 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -5,7 +5,6 @@
     | \.git
     | \.hg
     | \.mypy_cache
-    | \.tox
     | \.venv
     | _build
     | buck-out
diff --git a/requirements_dev.txt b/requirements_dev.txt
index 4dfb5e397..f90e6c59d 100644
--- a/requirements_dev.txt
+++ b/requirements_dev.txt
@@ -14,6 +14,5 @@ pytest>=7.2.0
 pytest-cov>=4.0.0
 pytest-runner>=6.0
 toml>=0.10.2
-tox>=3.27.1
 twine>=4.0.1
 wheel>=0.37.1
diff --git a/tox.ini b/tox.ini
deleted file mode 100644
index 955db570d..000000000
--- a/tox.ini
+++ /dev/null
@@ -1,47 +0,0 @@
-[tox]
-envlist = flake8, black, py38, py39, py310
-requires = tox-conda
-skip_missing_interpreters=true
-
-[travis]
-python =
-    3.10: py310
-    3.9: py39
-    3.8: py38
-
-[testenv:flake8]
-basepython = python
-deps = flake8
-commands = flake8 tiatoolbox tests
-
-[testenv:black]
-basepython = python
-deps = black
-commands = black --check tiatoolbox
-
-[testenv]
-setenv =
-    PYTHONPATH = {toxinidir}
-conda_deps=
-    openjpeg>=2.4.0
-conda_channels=
-    conda-forge
-passenv =
-    TRAVIS CI
-deps =
-    pytest-cov==3.0.0
-    pytest-runner==5.2
-    pytest==6.2.5
-    coverage==6.3.2
-    toml==0.10.2
-    -r{toxinidir}/requirements.txt
-; If you want to make tox run the tests with the same versions, create a
-; requirements.txt with the pinned versions and uncomment the following line:
-;     -r{toxinidir}/requirements.txt
-commands =
-    pip install -U pip
-    pytest --basetemp={envtmpdir} \
-           --cov=tiatoolbox --cov-report=term --cov-report=xml \
-           --capture=sys \
-           --durations=10 --durations-min=1.0 \
-           --maxfail=1